[Prospec] Product
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[Prospec] FGF 1 Rat
Product Information:
Catalogue number:
CYT-075
Description:
Fibroblast Growth Factor-acidic Rat Recombinant (FGF-1) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 141 amino acids and having a molecular mass of 15.9 kDa.
The FGF acidic is purified by proprietary chromatographic techniques.
Source:
Escherichia Coli.
Physical Appearance:
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:
Lyophilized at a concentration of 1 mg/ml in 5mM Na
2PO
4, pH-7.5 and 50mM NaCl.
Stability:
Lyophilized Fibroblast Growth Factor-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-a should be stored at 4°C between 2-7 days and for future use below -18°C. Please prevent freeze-thaw cycles.
Purity:
Greater than 98.0% as determined by SDS-PAGE.
Biological Activity:
The ED50 as determined by the dose-dependent proliferation of mouse BALB/c 3T3 cells, is less than 0.2ng/ml corresponding to a Specific Activity of 5*10
6IU/mg.
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